久久久久久av无码免费网站下载,一本加勒比hezyo无码资源网,久久久久黑人强伦姧人妻,好爽毛片一区二区三区四

技術文章您現(xiàn)在的位置:首頁 > 技術文章 > 恒遠產(chǎn)品文獻:植物蘋果酸,植物檸檬酸ELISA試劑盒引用文獻

恒遠產(chǎn)品文獻:植物蘋果酸,植物檸檬酸ELISA試劑盒引用文獻

更新時間:2020-12-28   點擊次數(shù):746次

【文獻標題】Phosphate regulates malate/citrate-mediated iron uptake and transport in apple

【作者】Jiu-Cheng Zhang,Xiao-Na Wang,Wei Sun,et.al

【作者單位】山東農(nóng)業(yè)大學(Shandong Agricultural University)

【文獻中引用產(chǎn)品】

植物蘋果酸(MALIC ACID)ELISA試劑盒

植物檸檬酸(Citric Acid)ELISA試劑盒

【關鍵詞】Low phosphate,Iron-deficiency,Absorption,Organic acids,Apple

【DOI】doi.org/10.1016/j.plantsci.2020.110526

【影響因子(IF)】5.90

出版期刊】《Plant Science》

【產(chǎn)品原文引用】

Rhizosphere organic acid efflux collection and measurement

The apple plants were treated in hydroponic nutrient solution for 4 days (pH = 5.8), and the nutrient solution was then dried to a powder in a -80℃ freeze dryer. The powder was dissolved with 5 ml of deionized water, the solution was then passed through a cation exchange resin and the filtrate was collected. After lyophilizing again, the sample was dissolved in 5 ml of deionized water and stored at -20℃. The organic acid efflux was analyzed via enzyme-linked immunosorbent assay (ELISA; Hengyuan, Shanghai, China). Organic acid was made into a solid-phase antibody, and horseradish peroxidase (HRP) was then combined with the organic acid antibody to form an antibody-antigenenzyme-labeled antibody complex. After thorough washing, the TMB substrate was added to develop the color, with the TMB converted to blue under the catalysis of HRP and finally converted to yellow under the action of acid. The shade of the color positively correlated with the organic acid concentration in the sample. Finally, the absorbance was measured using a microplate reader at a wavelength of 450 nm. The organic acid concentration was calculated from a standard curve.Effluent malate content = (337.57 ×A450 –9.0216) ×5 ×V / (134.09× M). Effluent citrate content = (506.82 ×A450 –17.148) ×5 ×V /(192.13 × M). (A: absorbance, V: volume of filtrate, M: fresh weight of apple root).

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務員!

更多公司福利請關注“恒遠生物”VX公眾號!

 

聯(lián)


亚洲熟妇无码一区二区三区导航| 国产成人午夜无码电影在线观看| 久久久久无码精品国产| 精品国产一区二区三区av片| 亚洲爆乳无码一区二区三区| 性一交一乱一伦一| 欧美国产激情18| 成人毛片18女人毛片免费| 麻豆人妻无码性色av专区| 欧美怡红院免费全部视频| 粗一硬一长一进一爽一a级| 在线观看免费人成视频| 大学生被内谢粉嫩无套| 久久亚洲av成人无码国产电影| 日韩在线永久免费播放| 美女张开腿黄网站免费| 在线亚洲人成电影网站色www| 比比资源先锋影音网| 亚洲av无码一区二区三区电影| 日本少妇高潮喷水xxxxxxx| 成年女人a毛片免费视频| 久久精品中文闷骚内射| 日韩午夜理论免费tv影院| 亚洲中文字幕久久精品无码a| 俺来也俺去啦最新在线| 国产精品亚洲а∨无码播放| 日本强好片久久久久久aaa| 国内揄拍国内精品少妇国语| 99精品久久久久久久婷婷| 人妻无码中文字幕| 人妻丰满熟av无码区hd| 成人小说亚洲一区二区三区| 三级理论中文字幕在线播放| 国产成人艳妇aa视频在线| 日本高清视频在线www色| 亚洲熟妇色xxxxx欧美老妇y| 久久亚洲私人国产精品| 熟妇人妻av无码一区二区三区| 最近免费中文字幕| 三上悠亚av影院在线看| 人与嘼交av免费|