久久久久久av无码免费网站下载,一本加勒比hezyo无码资源网,久久久久黑人强伦姧人妻,好爽毛片一区二区三区四

技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 恒遠產(chǎn)品文獻:蝦蛋白酶,淀粉酶,脂肪酶活性ELISA試劑盒引用文獻

恒遠產(chǎn)品文獻:蝦蛋白酶,淀粉酶,脂肪酶活性ELISA試劑盒引用文獻

更新時間:2021-01-08   點擊次數(shù):776次

【文獻標題】Dietary prebiotic inulin benefits on growth performance, antioxidant capacity, immune response and intestinal microbiota in Pacific white shrimp (Litopenaeus vannamei) at low salinity

【作者】Li Zhou,Huifeng Li,Jian G. Qin,et.al

【作者單位】海南大學(Hainan University)

【文獻中引用產(chǎn)品】

蝦蛋白酶(Protease)ELSIA試劑盒

蝦淀粉酶(Amylase)ELISA試劑盒

蝦脂肪酶(Lipase)ELISA試劑盒

【關(guān)鍵詞】Low salinity,Inulin,Growth performance,Antioxidant capacity,Intestinal microbiota,Litopenaeus vannamei

【DOI】doi.org/10.1016/j.aquaculture.2019.734847

【影響因子(IF)】3.42

出版期刊】《Aquaculture》

【產(chǎn)品原文引用】

2.7. Biochemical assays

The entire intestinal of three shrimps mixed as one sample, the hepatopancreas of one shrimp as one sample, each group have six samples. All samples were weighed and homogenized with 9 vol (v/w)of 0.86% physiological saline. Then, the homogenate was centrifuged at 2500 ×g at 4 °C for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instruction.Activities of intestinal proteaseamylase, and lipase were measured using an enzyme-linked immune sorbent assay (ELISA) kit (Shanghai Hengyuan Biotechnology Co, Ltd) (Li et al., 2019). Phenol oxidase (PO)activity in hepatopancreas was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Nanjing Jiancheng Institute, China).The contents of malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT), acid phosphatase (ACP) in hepatopancreas were measured by using specific commercial assay kits (Nanjing Jiancheng Institute, China) (Zhang et al., 2008). Results were recorded on a microplate reader (Epoch, BioTek, USA).Total SOD activity was determined by using WST-1 method. One unit of SOD activity was defined as the amount of enzyme required for 1 mg tissue proteins in 1 mL of a reaction mixture SOD inhibition rates to 50% as monitored at 550 nm.CAT activity was determined by using ammonium molybdate colorimetric method. One unit of CAT activity was defined as 1 mg tissue proteins consumed 1 μmol H2O2 at 405 nm for 60 s. MDA content was determined by using TBA method, which was measured at 532 nm. ACP activity was determined by using colorimetric method. One unit of ACP activity was defined as 1 mg tissue proteins produced 1 mg phenol at 37 °C for 30 min as monitored at 550 nm. Activities of protease, amylase, lipase and phenol oxidase were measured using the enzyme-linked immunosorbent assay (ELISA) and the absorbance (OD) was measured at 450 nm. Total protein content was measured by using Coomassie brilliant blue method.

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請關(guān)注“恒遠生物”VX公眾號!

聯(lián)


精品综合久久久久久97| 麻豆人妻无码性色av专区| 狠狠噜天天噜日日噜视频麻豆| 国产成人精品久久亚洲高清不卡| 制服丝袜中文字幕在线| 八区精品色欲人妻综合网| 成人午夜性a级毛片免费| 国产av旡码专区亚洲av苍井空| 国产免费av片在线观看| 无线乱码一二三区免费看| 天天躁夜夜躁狠狠是什么心态| 女人高潮久久久叫人喷水| 国产无套内射又大又猛又粗又爽| 狠狠噜天天噜日日噜视频麻豆| 久久久久无码精品亚洲日韩| 国产日韩综合一区二区性色av| 国产精品二区一区二区aⅴ污介绍 国产女人精品视频国产灰线 | av无码精品一区二区三区宅噜噜| 精品少妇无码av无码专区| 免费人成网站视频在线观看国内| www国产内插视频| 国产午夜视频在线观看| 国产手机在线αⅴ片无码观看| 熟妇与小伙子matur老熟妇e| 久久久久亚洲精品中文字幕| 免费看国产曰批40分钟| 丰满爆乳无码一区二区三区| 亚洲aⅴ在线无码播放毛片一线天| 国产成a人亚洲精v品无码性色| 人人妻人人澡人人爽精品日本| 18禁免费无码无遮挡不卡网站| 久久 国产 尿 小便 嘘嘘 | 老熟妻内射精品一区| 免费无码av片在线观看播放| 国产成人无码18禁午夜福利p| 天天做天天爱夜夜爽毛片毛片| 久久亚洲精精品中文字幕| 婷婷亚洲综合五月天小说| 内射后入在线观看一区| 国产精品亚洲一区二区三区在线| 少妇高潮惨叫久久久久久电影|