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技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 小鼠8羥基脫氧鳥苷,小鼠過氧化脂質(zhì)ELISA試劑盒引用文獻

小鼠8羥基脫氧鳥苷,小鼠過氧化脂質(zhì)ELISA試劑盒引用文獻

更新時間:2022-02-15   點擊次數(shù):933次

【文獻標題】Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation

【作者】Bing Liu,Jun-Li Wang,Xin-Mei Wang,et.al

【作者單位】北京農(nóng)業(yè)大學(Beijing University of Agriculture)

【文獻中引用產(chǎn)品】

小鼠8羥基脫氧鳥苷(8-OHdG)ELISA試劑盒

小鼠過氧化脂質(zhì)(LPO)ELISA試劑盒

【關(guān)鍵詞】Superovulatory times,Injury model,LBP,Remedy,Oxidative stress

【DOI】doi.org/10.1016/j.theriogenology.2020.01.048

【出版期刊】《Theriogenology》

【產(chǎn)品原文引用】

8-OHdG and LPO levels of the collected ovarian tissues were determined using an ELISA kit, according to the manu-facturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd,Shanghai, China). The collected mouse ovaries were cracked with lysate. Each 10 mL henylmethane-sulfonyl (PMSF) was added in 1 mL radio immuno precipitation assay (RIPA) as lysate. Every 1 g of tissue with 9 mL lysate, then coated with mouse antibody against 8-OHdG or LPO. After incubation and washing concentrations of 8-OHdG or LPO were determined as described previously .Intra-and inter-CVs: 8-OHdG (6.3%, 8.1%), LPO (5.2%, 7.4%).


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