久久久久久av无码免费网站下载,一本加勒比hezyo无码资源网,久久久久黑人强伦姧人妻,好爽毛片一区二区三区四

技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 恒遠(yuǎn)產(chǎn)品文獻(xiàn):多聚甲醛試劑引用文獻(xiàn)

恒遠(yuǎn)產(chǎn)品文獻(xiàn):多聚甲醛試劑引用文獻(xiàn)

更新時(shí)間:2021-01-11   點(diǎn)擊次數(shù):862次
【文獻(xiàn)標(biāo)題】Human umbilical cord mesenchymal stem cell-derived exosomes suppress dermal fibroblasts-myofibroblats transition via inhibiting the TGF-β1/Smad 2/3 signaling pathway
【作者】Jian Hu, Yuanwen Chen, Yubin Huang,et.al
【作者單位】深圳市寶安區(qū)人民醫(yī)院(The People’s Hospital of Bao’an Shenzhen)
【文獻(xiàn)中引用產(chǎn)品】
多聚甲醛
【關(guān)鍵詞】Human umbilical cord mesenchymal stem cells,Exosome, TGF-β1/Smad2/3 signaling pathway,Dermal ibroblasts Myofibroblast
【DOI】/doi.org/10.1016/j.yexmp.2020.104468
【影響因子(IF)】2.39
出版期刊】《Experimental and Molecular Pathology》
【產(chǎn)品原文引用】
The hUC-MSCs or fibroblasts in exponential phase were seeded at 1 × 105 cells/mL and 3 mL/well in a 6-well plate with a cover glass. After culture for 24 h at 37℃ with 5% CO2, culture medium was discarded, and cells were fixed with paraformaldehyde (Shanghai HengYuan 
Biological Technology Co., Ltd., Shanghai, China) for 10 min. After discarding the fixative solution, the cover glasses were rinsed by PBS 3 times, each for 3 min. PBS was then absorbed with absorbent papers, normal goat serum was dripped on the glass, and fibroblasts were sealed for 30min at room temperature. The sealing liquid was absorbed by absorbent papers without washes. After that, diluted primary antibodies (supplementary Table 1) were dripped into each cover glass, which was incubated in a wet box at 4℃ overnight. Afterwards, the fluorescent secondary antibody goat anti-rat IgG H&L (Alexa Fluor® 488) (1/200, ab150113) was added. Then the glass was dripped with 4',6-diamidino-2-phenylindole (DAPI) for nucleus staining, and sealed with sealing liquid containing anti-fluorescent quenching agent, and then observed under the fluorescence microscope. The above antibodies were all purchased from Abcam Inc. (Cambridge, MA, USA).
完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員!
更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)!

 

聯(lián)


无码一区二区三区| 国产成人午夜无码电影在线观看| 无码国产精成人午夜视频一区二区 | 又黄又爽又无遮挡免费的网站| 亚洲av无码片vr一区二区三区| 精品无码国产自产在线观看水浒传 | 狠狠躁夜夜躁人人爽天天不卡软件| 无码色av一二区在线播放| 久久久久99精品成人片试看| 国产av寂寞骚妇| 亚洲人成人无码www| 国产av人人夜夜澡人人爽| 强行交换配乱婬bd| 男女一边摸一边做爽爽| 国产在线观看无码免费视频| 女人张开腿让男人桶爽| 欧美丰满熟妇乱xxxxx网站| 成人妇女免费播放久久久| 久久综合伊人77777麻豆| 国产对白国语对白| 97久人人做人人妻人人玩精品| 九一九色国产| 国产青草视频在线观看| 日韩av无码久久一区二区| 国产乱人伦偷精品视频| 无套中出丰满人妻无码| 久久久精品波多野结衣| 色五月丁香五月综合五月| 蜜臀av午夜一区二区三区| 日日摸日日碰人妻无码| 精品国产午夜肉伦伦影院| 天堂草原电视剧在线观看图片高清| 亚洲aⅴ天堂av天堂无码麻豆 | 丰满少妇a级毛片野外| 久久亚洲av永久无码精品| a级毛片毛片免费观看久| 亚洲妇熟xxxx妇色黄| 性色av无码久久一区二区三区| 久久精品国产精品亚洲精品| 国产色视频一区二区三区qq号 | 精品深夜av无码一区二区老年|