久久久久久av无码免费网站下载,一本加勒比hezyo无码资源网,久久久久黑人强伦姧人妻,好爽毛片一区二区三区四

技術(shù)文章您現(xiàn)在的位置:首頁(yè) > 技術(shù)文章 > 恒遠(yuǎn)產(chǎn)品文獻(xiàn):生物素標(biāo)記的山羊抗兔抗體引用文獻(xiàn)

恒遠(yuǎn)產(chǎn)品文獻(xiàn):生物素標(biāo)記的山羊抗兔抗體引用文獻(xiàn)

更新時(shí)間:2021-01-11   點(diǎn)擊次數(shù):815次

【文獻(xiàn)標(biāo)題】Circular RNA-hsa-circ-0000670 promotes gastric cancer progression through the microRNA-384/SIX4 axis

【作者】Pengliang Liu, Shuang Cai, Nuo Li

【作者單位】中國(guó)醫(yī)科大學(xué)附屬第四醫(yī)院(the Fourth Affiliated Hospital of China Medical University)

【文獻(xiàn)中引用產(chǎn)品】

生物素標(biāo)記的山羊抗兔抗體

【關(guān)鍵詞】Hsa-circ-0000670,MicroRNA-384,Sine oculis-related homeobox 4,Gastric cancer

【DOI】doi.org/10.1016/j.yexcr.2020.112141

【影響因子(IF)】3.21

出版期刊《Experimental Cell Research》

【產(chǎn)品原文引用】

The tissues were fixed with 4% paraformaldehyde for 24 h, hydrated with 80%, 90%, 100% ethanol and n-butanol, embedded with paraffin, and cut into 5-μm-thick sections. After being heated at 60°C for 1 h, the sections were dewaxed with xylene, dehydrated with gradient ethanol (80%, 90%, 100%) and immersed in 3% H2O2 for 10 min. Antigen retrieval was performed, and the sections were then blocked with 100 μL 5% bovine serum albumin at 37°C for 30 min. Subsequently, the sections were incubated with 100 μL primary rabbit antibodies against SIX4 (1: 2000, ab176713), VEGF (1: 250, ab32152), and CD31 (1: 50, ab28364) overnight at 4°C followed by incubation with a secondary biotin-labeled goat anti-rabbit antibody (HY90046, Shanghai HengYuan Biological Co., Ltd., Shanghai, China) at 37°C for 30 min. Finally, the sections were stained with DAB chromogen (Beijing Bioss Biotechnology Co., Ltd., Beijing, China), and the cell nucleus was stained with hematoxylin for 5 min. The positive cells were stained in brown-yellow. Sections were visualized by Image-Proplus image analysis software (Media Cybernetics, Silver Springs, MD, USA). The target protein expression was evaluated by the proportions of positive cells in total cells detected from 5 randomly selected fields of view on every slide (200-fold magnification). Proportion scores were assigned as follows: positive tumor cells/total tumor cells > 10% positive (+), positive cells < 10% negative (-). 

完整版PDF文獻(xiàn)請(qǐng)咨詢(xún)?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)!

聯(lián)


激烈的性高湖波多野结衣| 国产麻豆md传媒视频| 无码人妻aⅴ一区二区三区| 少妇伦子伦精品无吗| 亚洲色欲一区二区三区在线观看| 精品久久无码中文字幕| 少妇人妻诗雨系列无删减| 久久精品国产9久久综合| 国产免费无码一区二区三区| 无遮挡边吃摸边吃奶边做 | 国产va在线观看免费| 朝鲜女人大白屁股ass| 青青草原综合久久大伊人精品| 国产丝袜美女| 色窝窝无码一区二区三区| 国产综合在线观看| 国产精品乱码久久久久久软件| 免费无码又爽又刺激网站| 大陆极品少妇内射aaaaa| 国产精品对白交换视频| 亚洲精品亚洲人成人网| 天天综合网网欲色| 色偷偷人人澡人人爽人人模| 日日av拍夜夜添久久免费| 狠狠色噜噜狠狠亚洲av| 永久免费精品精品永久-夜色| 成人国产欧美大片一区| 男女激烈床震gif动态图免费| 久久精品国产亚洲夜色av网站| 麻豆人妻少妇精品无码专区| 成人无码视频| 尤物在线精品视频| 与子敌伦刺激对白播放| 亚洲国产精品无码久久| 国产精品成熟老女人| 欧美老妇交乱视频在线观看| 爽爽精品dvd蜜桃成熟时电影院| 一本久久a久久精品亚洲| 亚洲精品无码专区久久| 无码人妻一区二区三区在线| 女邻居丰满的奶水|